Early Intracellular Events in the Replication of T4 Bacteriophage Deoxyribonucleic Acid
نویسندگان
چکیده
منابع مشابه
Intracellular inactivation of bacteriophage T4 early deoxyribonucleic acid.
As previously shown, a small amount of polynucleotide material is added to parental T4 deoxyribonucleic acid (DNA) molecules within the first 5 min of infection. I have asked whether this process is essential for phage replication. Two approaches-one involving decay of (32)P incorporated into this "early DNA" and the other involving photoinactivation of bromodeoxyuridine-containing early DNA-in...
متن کاملRole of genes 46 and 47 in bacteriophage T4 reproduction. I. In vivo deoxyribonucleic acid replication.
Functional proteins coded by genes 46 and 47 are required for (i) continuation of deoxyribonucleic acid (DNA) synthesis in the late period of T4 infection and (ii) production of normal, late replicating DNA which contains strands with a sedimentation coefficient in alkaline sucrose greater than that of mature DNA (73S). Continued DNA synthesis in the late period in the absence of functional gen...
متن کاملEqual incorporation of both parental bacteriophage T7 deoxyribonucleic acid strands into intracellular concatemeric deoxyribonucleic acid.
After infection of Escherichia coli B with radiolabeled T7 bacteriophage, the parental deoxyribonucleic acid label was found in both polynucleotide chains of the intracellular T7 concatemer.
متن کاملInhibition of host deoxyribonucleic acid synthesis by T4 bacteriophage in the absence of protein synthesis.
The requirement for phage protein synthesis for the inhibition of host deoxyribonucleic acid synthesis has been investigated by using a phage mutant unable to catalyze the production of any phage deoxyribonucleic acid. It has been concluded that the major pathway whereby phage inhibit host syntheses requires protein synthesis. The inhibition of host syntheses by phage ghosts is not affected by ...
متن کاملProperties of bacteriophage T4 mutants defective in gene 30 (deoxyribonucleic acid ligase) and the rII gene.
In Escherichia coli K-12 strains infected with phage T4 which is defective in gene 30 [deoxyribonucleic acid (DNA) ligase] and in the rII gene (product unknown), near normal levels of DNA and viable phage were produced. Growth of such T4 ligase-rII double mutants was less efficient in E. coli B strains which show the "rapidlysis" phenotype of rII mutations. In pulse-chase experiments coupled wi...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1970
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.5.4.533-535.1970